Asuragen – AmplideX Nanopore Carrier Plus
Introducing the Asuragen AmplideX® Nanopore Carrier Plus Kit* – a revolutionary solution for carrier screening for challenging hard-to-read genes, leveraging long-read sequencing technology to provide unparalleled insights into genetic conditions. Expanded prenatal screening protocols traditionally require multiple methods to ensure full coverage of genes of interest. Carrier Plus, partnered with Oxford Nanopore, provides one platform to cover multiple variant types across key genes. It can be used as a primary genetic analysis method or to complement existing NGS workflows. Covering 11 genes of interest FMR1, CFTR, SMN1/2, HBA1/2, HBB, F8 Inversions, GBA, CYP21A2, TNXB.
Carrier Plus key benefits:
- Comprehensive Coverage:
- Screens for nine high-prevalence carrier conditions across 11 genes, ensuring thorough genetic analysis
- Enhanced Insight:
- Utilises long-read sequencing to detect complex genetic variations that are often missed by short-read sequencing methods eg. AGG interruptions in FMR1, reducing the need for reflex testing
- Consolidated Workflow:
- A single, flexible workflow designed to streamline and consolidate existing assays and methods, reducing the need for multiple tests
- Customised Design:
- Features four mixes across the 11 genes, allowing for customisable and scalable testing options. Use just the tubes you need. Can be purchased separately
- Integrated Data Analysis:
- The AmplideX Reporter software simplifies data analysis and reporting, providing a seamless and efficient workflow
Why Choose the Asuragen Carrier Plus Kit?
- Reliable Results: Achieve consistent and accurate results with our state-of-the-art long-read sequencing technology partnered with Oxford Nanopore.
- User-Friendly: Simplified protocols and optimized reagents make the Asuragen Carrier Plus Kit easy to use, even for those new to carrier screening.
One Workflow, 9 High-Prevalence Carrier Screening Conditions, 11 Genes
Figure 1 – High level assay workflow
Figure 2A. Composition of 11 genes across 4 tubes
AmplideX Nanopore Carrier Plus Kit Proposed Product Features
| Sample Type | Whole blood, cheek swab, saliva |
| Extraction | Multiple automated and manual methods, 20-80 ng input per PCR reaction |
| Workflow | Up to 4 PCRs per sample, 1 library prep per sample, 1 instrument for key hard-to-decipher genes |
| Throughput | Up to 96 samples per MinION flow cell |
| Instruments | Various thermocycler models; ONT sequencers (Mk1B, GridION), R10.4.1 flow cells and reagents; will support Q-Line |
| Software | Amplidex Reporter interface with ONT instrument setup/execution and analysis |
* Product is for research use only (RUO)
THESE PRODUCTS ARE NOT AVAILABLE FOR PURCHASE BY THE GENERAL PUBLIC.
