SEBIA: Capillary Electrophoresis

Products are for professional/laboratory use only.  Sebia are world leaders in Electrophoresis testing. Their capillary electrophoresis [CE] technology leads the market with systems to suit both small and large laboratories; along with a large test menu.

Their systems utilise the proven, high performance technology of capillary electrophoresis for clear separation of analytes. They feature whole blood analysis, with no pre-analytical preparation; cap-piercing for improved workflow.

Technology Principle

The Sebia systems use the principle of capillary electrophoresis in free solution.

Charged molecules are separated by their electrophoretic mobility at a specific pH in an alkaline buffer. Separation occurs according to the electrolyte pH and electroosmotic flow.

The Sebia capillary electrophoresis instruments are equipped with several parallel capillaries enabling multiple simultaneous analyses:

• CAPILLARYS 3 TERA (12 capillaries)
• CAPILLARYS 3 Octa (8 capillaries)

​Each sample is diluted in a dilution buffer and the capillaries are filled with the separation buffer; samples are then injected by aspiration into the anodic end of the capillary. A high voltage protein separation is then done; direct detection and quantification of the different protein fractions is performed at a specific wavelength at the cathodic end of the capillary.

Post analysis, the capillaries are immediately cleaned with a wash solution and then refilled with buffer in preparation for the next samples.

Comprehensive CE Menu:

• Hb A1c venous & capillary blood
• Haemoglobin
• Serum Protein and Immunotyping
• Urine Proteins and Immunotyping
• NEONAT Hb
• CDT/ CDT_IFCC

Capillary Electrophoresis Instruments:

• Capillarys 3 Octa 
  –  8 Capillaries

• Capillarys 3 Tera
  –  12 Capillaries

• Capillarys 3 Tera MC configuration
  –  Workcell with up to 3 Capillarys 3 Tera instruments attached.

Technical Characteristics

• Positive ID & Reagent Traceability
  – Reagent management through RFID tags
• Batch optimisation
• Continuous sample loading
• Efficient mixing system with multiple sample inversions (HbA1c & Hb)
• Sampling from open and capped tubes (cap piercing)
• Sample type
  – Serum
  – Urine
  – Whole blood (capped tubes), venous blood, capillary blood samples
  – Cord blood
  – Dry blood spots
• Tube capacity: from 120 samples with the onboard loading to 600 samples with the additional tube loader
• 24/7 accessibility
• Automatic start-up / shut down
• Automatic maintenance

PHORESIS CORE Software:

• Interpretation and results validation
• Multi-criteria search function; including patient result history
• SQL Client/Server database
  – Networking of all Sebia Instruments, within a laboratory and between distant sites
  – Decentralised validation
  – Unlimited storage capacity of patient database

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