Luminex – xMAP Microspheres

xMAP^® microspheres (or beads) come in a variety of formats. MagPlex^® Microspheres, for example, are 6.5 micron superparamagnetic beads that are dyed with up to three red and infrared fluorescent dyes, resulting in 500 distinctly colored bead sets and delivering data on up to 500 analytes per well.

Get Data on up to 500 Analytes per Well

Multiplexing biological assays reduces time to results, sample requirements, and costs compared to traditional methods. With an open architecture design, you can choose from an extensive menu of commercially available kits and design your own assays.

Microsphere Analysis

xMAP beads are internally color-coded microspheres with precise concentrations of multiple fluorescent dyes, yielding up to 500 distinctly colored bead sets.

xMAP microspheres can be coupled with target-specific molecules such as antigens, antibodies, oligonucleotides, enzyme substrate, receptors, etc. After an analyte in a sample is captured by this target-specific molecule, a fluorescent reporter molecule is introduced to complete the reaction on the surface of each microsphere.

The internal dyes on the microspheres are excited by the red laser or LED to identify the microsphere set. Then, a green laser or LED excites the fluorescent reporter dye.

Using these signals, each individual microsphere is identified, and the amount of target molecule bound to its surface is quantified.

How It Works

Approximately 10 surface carboxyl groups (COOH) cover the surface of each bead and serve as covalent attachments for capture ligands. A fluorescent reporter is coupled to a target molecule, enabling detection following capture on the microsphere’s surface.

Advantages and Benefits

• High-Throughput Testing and Analysis – Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously.
• Versatility – A single xMAP system can perform assays using several different methods and chemistries, including nucleic acid hybridization, antigen-antibody binding, enzyme reactivity, receptor-ligand binding, and other protein interactions.
• Accuracy – xMAP Technology generates data and accurately quantifies the biological interactions being tested.
• Flexibility – Multiplexing supports a variety of applications ranging from life sciences research and clinical diagnostics to food safety and biodefense.
• Reproducibility – High-volume production of xMAP microspheres within a single lot delivers reduced variability that solid-phased flat arrays cannot provide.

MagPlex^® Microspheres at a Glance:

• Surface carboxyl groups
• Superparamagnetic
• 500 regions available
• Compatible with all xMAP instruments
• Multiple concentrations available supporting both small scale and high throughput applications
• Same reliable coupling chemistry as MicroPlex microspheres
• Dramatically improved washing efficiency
• Ideal for automated applications
• No need for expensive filter plates

MagPlex-TAG^™ Microspheres at a Glance:

• 150 unique 24-mer surface anti-TAG DNA sequences
• 150 regions available (each with unique anti-TAG)
• Compatible with all xMAP instruments
• 2.5 million beads/mL
• Proven xTAG® Technology
• Improved washing efficiency
• Ideal for automated applications
• No need for expensive filter plates or oligo coupling
• Convenient 2.5 million bead/mL concentration for improved handling

MagPlex-Avidin Microspheres at a Glance:

• Avidin protein-coated surface
• 100 regions available
• Compatible with all xMAP instruments
• 2.5 million beads/mL
• Near-covalent biotin-binding chemistry
• Ideal for coupling small molecules, like biotinylated peptides
• Suitable for protein and immunogenicity applications
• Superparamagnetic
• No need for expensive filter plates

MagPlex Monitoring Microspheres at a Glance:

• Superparamagnetic
• MagPlex RP1 Monitor Beads are compatible with all xMAP instruments
• MagPlex RP2 Monitor Beads are compatible with new violet channel on xMAP INTELLIFLEX DR-SE
• Available at the same concentration as region beads (12.5 million beads/mL)
• Well-to-well assay performance monitoring
• Compatible with proteomic and genomic applications
• Eliminate instrument or user errors as source of unexpected results

MicroPlex^® Microspheres at a Glance:

• Surface carboxyl groups
• 100 regions available
• Compatible with Luminex^® 200™, FLEXMAP 3D^®, and xMAP INTELLIFLEX^®
• Multiple vial sizes available supporting both small scale and higher-throughput applications.
• 12.5 million beads/mL
• Suitable for both protein and nucleic acid applications

LumAvidin^® Microspheres at a Glance:

• Avidin protein-coated surface
• 100 regions available
• Compatible with Luminex 200™, FLEXMAP 3D, and xMAP INTELLIFLEX
• 1 mL vial size
• 2.5 million beads/mL
• Near-covalent biotin-binding chemistry
• Ideal for coupling small molecules, like biotinylated peptides
• Suitable for protein and immunogenicity applications
• No need to couple avidin to beads

SeroMAP™ Microspheres at a Glance:

• Specifically designed for protein-based serological applications
• 100 regions available
• Compatible with Luminex 200™, FLEXMAP 3D, and xMAP INTELLIFLEX
• 4 mL vial size only
• 12.5 million beads/mL
• Lower non-specific binding

Learn more about Luminex xMAP Microspheres

THESE PRODUCTS ARE NOT AVAILABLE FOR PURCHASE BY THE GENERAL PUBLIC.

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