TriLink - CleanCap M6 mRNAs

30Apr

TriLink – CleanCap M6 mRNAs

Last updated: 01st May, 2024

THESE PRODUCTS ARE NOT AVAILABLE FOR PURCHASE BY THE GENERAL PUBLIC.

Three mRNA designs for diverse projects. TriLink offers premade mRNAs in three designs: CleanCap^® mRNAs, CleanCap mRNAs (5moU), and CleanCap M6 mRNAs (N1MePsU). They all contain an optimal 5′ Cap 1 found in higher eukaryotes for their functionality and stability.

Their 5′ UTR is synthetic, containing a strong Kozak sequence, designed to promote efficient translation while the 3′ UTR is derived from mouse alpha-globin. Their key differences lie in the type of CleanCap analog used and the base compositions, which may affect their protein expression and immunogenicity. View the three TriLink CleanCap options in the table below.

	CleanCap mRNAs	CleanCap mRNAs (5moU)	CleanCap M6 mRNAs (N1MePsU)
Expression system	Mammalian	Mammalian	Mammalian
5′ cap	Cap 1	Cap 1	Cap 1
Cap analog	CleanCap AG	CleanCap AG	CleanCap M6
5′ and 3′ UTRs	Included	Included	Included
Poly(A) tail	120 nt	120 nt	120 nt
Sequence composition	Unmodified uridines	Uridines substituted with 5-methoxyuridine	Uridines substituted with N1-methylpseudouridines
Protein expression	High	High	Highest
Immunogenicity	Normal	Reduced	Lowest

Six new CleanCap mRNA constructs

The new constructs are available in three sizes (100 µg, 1 mg, 5 mg) and bulk. These constructs are listed in the table below.

Gene	Cat no. / ordering page	Supporting documents
Enhanced green fluorescent protein (EGFP)	L-8101	Product insert
Firefly luciferase (FLuc)	L-8102	Product insert
mCherry	L-8103	Product insert
CRISPR Associated Protein 9 (Cas9)	L-8106	Product insert
Erythropoietin (EPO)	L-8109	Product insert
Cre recombinase (Cre)	L-8111	Product insert